PoplarV1, Main, Exploration, bibRecord, 003A08

A modified T-vector for simplified assembly of hairpin RNAi constructs.

Identifieur interne : 003A08 ( Main/Exploration ); précédent : 003A07; suivant : 003A09

A modified T-vector for simplified assembly of hairpin RNAi constructs.

Auteurs : Keming Luo [États-Unis] ; Scott A. Harding ; Chung-Jui Tsai

Source :

Biotechnology letters [ 0141-5492 ] ; 2008.

RBID : pubmed:18317701

Descripteurs français

KwdFr :
- ARN des plantes (génétique), Alcohol oxidoreductases (génétique), Clonage moléculaire (méthodes), Gènes rapporteurs (génétique), Interférence par ARN (MeSH), Populus (génétique), Protéines végétales (génétique), Protéines à fluorescence verte (génétique), Séquences répétées d'acides nucléiques (génétique), Vecteurs génétiques (génétique).
MESH :
- génétique : ARN des plantes, Alcohol oxidoreductases, Gènes rapporteurs, Populus, Protéines végétales, Protéines à fluorescence verte, Séquences répétées d'acides nucléiques, Vecteurs génétiques.
- méthodes : Clonage moléculaire.
- Interférence par ARN.

English descriptors

KwdEn :
- Alcohol Oxidoreductases (genetics), Cloning, Molecular (methods), Genes, Reporter (genetics), Genetic Vectors (genetics), Green Fluorescent Proteins (genetics), Plant Proteins (genetics), Populus (genetics), RNA Interference (MeSH), RNA, Plant (genetics), Repetitive Sequences, Nucleic Acid (genetics).
MESH :
- chemical , genetics : Alcohol Oxidoreductases, Green Fluorescent Proteins, Plant Proteins, RNA, Plant.
- genetics : Genes, Reporter, Genetic Vectors, Populus, Repetitive Sequences, Nucleic Acid.
- methods : Cloning, Molecular.
- RNA Interference.

Abstract

We describe a modified T-vector, pGFPm-T, for direct cloning of RT-PCR products to generate bidirectional restriction fragments for assembly of hairpin-containing RNAi vectors in the popular pFGC and pGSA binary vector backbone. Green fluorescence protein (GFP) is used as a visual reporter for direct selection of recombinants under UV illumination. The simplified cloning process enables a seamless workflow from candidate gene selection and RT-PCR verification to inverted repeat cloning, using a single pair of gene-specific primers.

DOI: 10.1007/s10529-008-9673-x
PubMed: 18317701

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name>
<affiliation wicri:level="2"><nlm:affiliation>Biotechnology Research Center, School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931, USA.</nlm:affiliation>
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<author><name sortKey="Harding, Scott A" sort="Harding, Scott A" uniqKey="Harding S" first="Scott A" last="Harding">Scott A. Harding</name>
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<term>Genes, Reporter (genetics)</term>
<term>Genetic Vectors (genetics)</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Plant Proteins (genetics)</term>
<term>Populus (genetics)</term>
<term>RNA Interference (MeSH)</term>
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<term>Repetitive Sequences, Nucleic Acid (genetics)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (génétique)</term>
<term>Alcohol oxidoreductases (génétique)</term>
<term>Clonage moléculaire (méthodes)</term>
<term>Gènes rapporteurs (génétique)</term>
<term>Interférence par ARN (MeSH)</term>
<term>Populus (génétique)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines à fluorescence verte (génétique)</term>
<term>Séquences répétées d'acides nucléiques (génétique)</term>
<term>Vecteurs génétiques (génétique)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Alcohol Oxidoreductases</term>
<term>Green Fluorescent Proteins</term>
<term>Plant Proteins</term>
<term>RNA, Plant</term>
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<term>Genetic Vectors</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term>
<term>Alcohol oxidoreductases</term>
<term>Gènes rapporteurs</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Protéines à fluorescence verte</term>
<term>Séquences répétées d'acides nucléiques</term>
<term>Vecteurs génétiques</term>
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<front><div type="abstract" xml:lang="en">We describe a modified T-vector, pGFPm-T, for direct cloning of RT-PCR products to generate bidirectional restriction fragments for assembly of hairpin-containing RNAi vectors in the popular pFGC and pGSA binary vector backbone. Green fluorescence protein (GFP) is used as a visual reporter for direct selection of recombinants under UV illumination. The simplified cloning process enables a seamless workflow from candidate gene selection and RT-PCR verification to inverted repeat cloning, using a single pair of gene-specific primers.</div>
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A modified T-vector for simplified assembly of hairpin RNAi constructs.

A modified T-vector for simplified assembly of hairpin RNAi constructs.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki